Performance evaluation of UV/H2O2 process applied to treat chlorpyrifos ethyl in aqueous solution: Investigation of the genotoxicity using Single Cell Gel Electrophoresis Assay

H. Djelal, M. Chaouch, N. Mustapha, C. Recordel-Henriel, H. Ben Mansour


Abstract: This study aimed at evaluating the performance of chlorpyrifos ethyl degradation (CP) by a 254-nm ultraviolet irradiation (UV) or in the presence of hydrogen peroxide (H2O2) or during a UV/H2O2 process. The degradation rates of chlorpyrifos ethyl, an organophosphate (OP) used in agriculture, were respectively 24 %, 83% and 93%. With the three types of treatments, the degradation of this organophophate showed a pseudo first-order kinetic pattern. The advanced oxidation process with a UV/H2O2 treatment was found to be a technically feasible approach for the removal of chlorpyrifos ethyl. In addition, the genotoxicity of the studied compound, before and after several treatments, was evaluated in vivo in the Mediterranean wild mussels – the Mytilus galloprovincialis - using assay comet for the quantification of DNA fragmentation. The percentage of genotoxicity was 42% and 43% after an exposure to 0.66 mg kg-1 body weight (bw) and a 0.33 mg kg-1 bw of CP, respectively. The genotoxicity decreased at 20% when tested with the CP obtained after a UV/H2O2 treatment. However, after a four-day biological treatment with Pseudomonas peli, we observed a rate of degradation of 98% and the percentage of genotoxicity was 8% and 15% after an exposure to 0.66 mg kg-1 bw and a 0.33 mg kg-1 bw of CP, respectively.

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