Recovery of proteins from EDAM whey using membrane ultrafiltration

F. Yelles- Allam, A. Nouani


Abstract : In Algeria, whey is discharged without any treatment and this causes not only pollution problem, but also a loss in nutritive components of milk. In this paper, characterization of EDAM whey is done, which is resulted from pasteurised mixture of cow’s milk and skim milk. The recovery of whey protein by ultrafiltration/diafiltration, was studied. The physical-chemical analysis of whey has emphasized on its pollutant and nutritive characteristics. In fact, its BDO5 and CDO are 49.33, and 127.71 g of O2/l of whey respectively. It contains: fat (1,90±0,1  gr/l), lactose (47.32±0,57 gr/l), proteins (5,82±0,4gr/l) and ashes 4.53±0.15gr/l, calcium (0,518gr/l), Na (1.104gr/l), K (1.014 gr/l), Mg (0.118 gr/l) and P (0.482 gr/l). Tangential ultrafiltration of whey after treatment was carried out in a polyetersulfone membrane with a cut-off of 10K. Its hydraulic intrinsic resistance (Rm) and permeability (Lp) are respectively: 2.041.1012 m-1 and 176, 32 l/h.m2bar. The dry matter obtained at FC6 is of 70.25 g/l, containing 16.33g/l of proteins. The retention rate of protein is 98.18 % and the decrease in BDO5 and CDO are at 18.875 and 42.818 g of O2/liter of permeate respectively. Diafiltration performed on concentred retentat allowed the complete removal of lactose and minerals. The ultrafiltration of the whey before the disposal is an alternative for Algéria dairy industry.

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G. Daufin, F .René and P.Aimar, Les separations par membrane dans les procédés de l'industrie alimentaire. Paris, France: Collection Sciences et Techniques Agroalimentaires; 1998.

Rektor, G. Vatai, Membrane filtration of Mozzarella whey, Desal. 162

(2004) 279–286.

E. Suárez , A. Lobo , S.Álvarez , F. A. Riera and R. Álvarez, Partial demineralization of whey and milk ultrafiltration permeate by nanofiltration at pilot-plant scale Desalination 198(2006) 274–281

P. Fox, T.Guinee, T. Cogan, McSweeney P (2000). Fundamentals of Cheese Science. USA: Aspen Publishers Inc.

G.Mockaitis, S.M.Ratusznei, Jose.A.D .Rodrigues, M. Zaiat and E. Foresti, Anaerobic whey treatment by a stirred sequencing batch reactor (ASBR): effects of organic loading and supplemented alkalinity. J. Environ. Manage. 79, (2006) 198–206.

H.Gannoun, E. Khelifi, H. Bouallagui, Y. Touhami and M. Hamdi, Ecological clarification of cheese whey prior to anaerobic digestion in up flow anaerobic filter. Bioresource. Technology 99, (2008) 6105–611.

W.S.GeoffreyWhey and whey proteins—From ‘gutter-to-gold’, International Dairy Journal 18 (2008) 695–704.

J.L. Maubois,. Techniques séparatives appliquées à la valorisation du lait. I.A.A. déc, 12 (2000) pp 32-36.

N.S.P.S.Richards , Soro Lácteo, Perpectivas Industriais e Proteção ao Meio Ambiente, Food Ingred. (2002) 17 20–27.

F.Yelles, A. Nouani, A. Kahlouche and S.Belbraouet, Whey Ultrafiltration: Effect of pH on Permeate Flux and Proteins Retention. World Applied Sciences Journal 33 (5) (2015) 744-751.

Afnor, 1986. Methods of analysis of milk and dairy products. Collection of French standards, 2 edition, 580p.

O.Lowry, N.J. Rosebrough and A.L. Farrand and R.J. Randal, Protein measurement with Folin phenol reagent. J. Biol. Chem., 193 (1951) 267-275.

C. Audigui, J. Figarella and F. Zonszan, Manipulation of biochemical analysis, the first edition, Paris, (1984) 88-95.

P. Franck .Rejsek , Analyse des eaux ; aspects réglementaires et techniques ; centre Régional de documentation pédagogique d’aquitaine, ,(2002) 357p.

J. Rodier, C .Bazin, J.P Broutin , P.Chambon, H.Champsaur and L.Radi , L'analyse de l'eau; eaux naturelles, eaux résiduaires, eaux de mer.8ieme édition DUNOD, Paris(1996) .

W.S .Geoffrey, Symposium: Advances in Dairy foods processing and engineering .Journal of Dairy Science Vol. 79, No. 8. (1996).

Y.M.Lo, S.T.Yang and D.B.Min, Ultrafiltration of xanthan gum fermentation broth: Process and economic analyses. J. Food. Eng.31 (2),( 1997) 219–236.

Su-Hsia Lin, Chia-Lin Hung and Ruey-Shin Juang.Applicability of the exponential time dependence of flux decline during dead-end ultrafiltration of binary protein solutions. Chemical Engineering Journal 145(2008) 211–217.

C. Baldasso ⁎ , T.C. Barros and I.C. Tessaro, Concentration and purification of whey proteins by ultrafiltration .Desalination 278 (2011) 381–386.

L.M .Eugenia, S.Alvarez , C.F.A.Menendez, R.B.Alvarez Ri, β-Lactoglobuline removal from whey protein .Concentrates production of milk derivatives as a base for infant formulas. Separation and Purification Technology ; 52 (2006 ) pp 310- 3.

M. R.Etzel, Manufacture and use of dairy protein fractions. Journal of Nutrition,134, (2004). 996S-1002S.


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