Protective effect of olive mill wastewater against lipid metabolism perturbation induced in cafeteria diet fed rats

D. Messaoudi, Z. Bouallagui, A. Henab, F. Moulti-Mati


Abstract: The biological activities of phenolic compounds from olive mill wastewater (OMWW) have been extensively studied. However, the bioactivity of raw OMWW has not been yet well demonstrated in vivo. The objective of this study was to evaluate the effect of oral administration of OMWW on cafeteria diet induced metabolic disorders in rats. Our results showed that within a 13-week treatment period, the cafeteria diet induced an increase in glucose and triglycerides levels. The levels of glucose, triglycerides and the total cholesterol were significantly decreased after supplementation with OMWW (p < 0.05). To evaluate the toxicity effect of OMWW, the results concerning the blood parameters of the renal and liver function (urea, creatinine, uric acid, bilirubin, total protein and transaminases) showed that there are not difference between the control and the OMWW supplementation groups. The liver histology from rats fed with cafeteria diet showed a steatosis which is characterized by lipid droplet accumulation in the hepatocytes cytoplasm. In OMWW supplemented groups, the liver tissue showed a structure similar to the control group.  HPLC analysis of OMWW reveled the major presence of a phenolic acid wich is hydroxytyrosol. In conclusion, our study suggests that OMWW brought real health benefits by protecting liver tissue and modulating some biological parameters such as lipid parameters.

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Haslam, D.W.; James, W.P. Obesity. Lancet 366 (2005) 1197–209.

Keys, A. Mediterranean diet and public health: personal reflections. The American Journal of Clinical Nutrition 61(1995) 1321-1323.

Rodis, P.S.; Karathanos, V.T.; Mantzavinou, A.

Partitioning of olive oil antioxidants between oil and water phases. Journal of Agricultural and Food Chemistry 50(2002) 596– 601.

Capasso, R. The chemistry, biotechnology and

ecotoxicology of the polyphenols naturally occurringin vegetable wastes. Current Topics in Phytochemistry Research Trends1 (1997) 145-156.

Greco, G.J.; Colarieti, M.L.; Toscano,G.; Iamarino, G.;Rao, M.A.; Gianfreda, L. Mitigationof olive mill wastewater toxicity. Journal of Agricultural and Food Chemistry54(2006) 6776-6782.

Servilli, M.; Baldioli, M.; Selvaggini, R.; Minitiati, E.; Macchioni, A.; Montedoro, G.F. HPLC evaluation of phenols in virgin olive oil, vegetation waters and pomace and 1D- and 2D-NMR characterization. Journal of the American Oil Chemist’s Society 76 (1999) 873-882.

Visioli, F.; Romani, A.; Mulinacci, N.; Zarini, S.; Conte, D.; Vincieri, F.F.; Galli, C. Antioxidant and other biological activities of olive mill waste waters. Journal of Agricultural and Food Chemistry47(1999) 3397-3401.

Gorinstein,S.; Leontowicz,H.; Lojek, A.; Leontowicz,

M.; Ciz, M.; Krzeminski,R.; Gralak, M.; Czerwinski, J.; Jastrzebski, Z.; Trakhtenberg, S.; Grigelmo-Miguel, N.; Soliva-Fortuny, R. Olive oils improve lipid metabolism and increase antioxidant potential in rats fed diets containing cholesterol. Journal of Agricultural and Food Chemistry 50(2002)6102-6108.

Petroni, A.; Blasevich, M.; Salami, M.; Papini, N.,

Montedero,G.F.; Galli, C. Inhibition of platelet aggregation and eicosanoid production by phenolic components of olive oil. Thrombosis Research78 (1995) 151-160.

De la Puerta, R.; Ruiz-Gutierrez, V.; Hoult, J.R.

Inhibition of leukocyte 5-lipoxygynase by phenolics from virgin olive oil. Biochemical Pharmacology57 (1999) 445-449.

Hamden, K.; Allouche, N.; Damak, M.; Elfeki, A.

Hypoglycemic and antioxidant effects of phenolic extracts and purified hydroxytyrosol from olive mill waste in vitro and in rats. Chemico-Biological Interactions 180(2009) 421-432.

Hamden, K.; Allouche, N.; Jouadi, B.; El-Fazaa, S.; Gharbi, N.; Carreau, S.; Damak, M.; Elfeki, A. Inhibitory Action of Purified Hydroxytyrosol from Stored Olive Mill Waste on Intestinal Disaccharidases and Lipase Activities and Pancreatic Toxicity in Diabetic Rats. Food Science and Biotechnology19 (2010) 439-447.

Cao, K.; Xu, J.; Zou, X.; Li, Y.; Chen, C.; Zheng, A.

. Hydroxytyrosol prevents diet-induced metabolic syndrome and attenuates mitochondrial abnormalities in obese mice. Free Radical Biology and Medicine 67(2014) 396-40.

Dubois,M.; Gilles, K.; Hamilton, J.K.; Rebers, P.A.;

Smith,F. A colorimetric method for the determination of sugars. Nature 4265(1951)168- 167.

Koop, D.R.; Klopfenstein, B.; Iimuro,Y.; Thurman, R.G. Gadolinium chloride blocks alcohol-dependent liver toxicity in rats treated chronically with intragastric alcohol despite theinduction of CYP2E1.Molecular Pharmacology51(1997) 944-950.

Slaughter,T.N.; Paige,A.; Spires,D.; Kojima, N.; Kyle, P.B.; Garrett, M.R. et al. Characterization of the development of renal injury in Type-1 diabetic Dahl salt-sensitive rats. American journal of physiology Regulatory, integrative and comparative physiology 305(2013) 727-734.

Fki, I.; Sahnoun, Z.; Sayadi, S. Hypocholesterolemic

effects of phenolic extracts and purified hydroxytyrosol recovered from olive mill wastewater in rats fed a cholesterol-rich diet.Journal of Agricultural and Food Chemistry55 (2007) 624-31.

Bouaziz, M.; Hammami, H.; Bouallagui, Z.; Jemai,

H.; Sayadi, S. Production of antioxidants from olive processing by-products. Electronic journal of environmental, agricultural and food chemistry 7(2008) 3231-3236.

Chung, H.Y.;Yoro, M.K.; Kawagishi, H. Characteristics of water-soluble polysaccharide, showing inhibiting activity on α-glucosidase, in Cordycepsmilitaris. Food Science and Biotechnology 18(2009) 667-671.

Adisakwattana, S.; Charoenlertkul, P.; Yibchok-Anun, S.α-Glucosidase inhibitory activity of cyanidin-3- galactoside and synergistic effect with acarbose. Journal of Enzyme Inhibition and Medicinal Chemistry 24(2009) 65-69.

Jemai, H.; Fki, I.; Bouaziz, M.; Bouallagui, Z.; El

Feki, A.; Isoda, H.; Sayadi, S. Lipid-Lowering and Antioxidant Effects of Hydroxytyrosol and Its Triacetylated Derivative Recovered from Olive Tree Leaves in Cholesterol-Fed Rats.Journal of Agricultural and Food Chemistry 56(2008) 2630–2636.

Fki, I.; Bouaziz, M.; Sahnoun, Z.; Sayadi, S.

Hypocholesterolemic effects of phenolic-rich extracts of Chemlali olive cultivar in rats fed a cholesterol-rich diet. Bioorganic and Medicinal Chemistry 13(2005) 5362-70.

Priore, P.; Cavallo, A.; Gnoni, A.;Damiano, F.;Gnon,

G. V.; Siculella, L. Modulation of hepatic lipid metabolism by olive oil and its phenols in nonalcoholic fatty liver disease. International Union of Biochemistry and Molecular Biology Life 67(2015) 9-17.

Rincon-Cervera, M.A.;Valenzuela, R.; Hernandez-Rodas, M.C.; Marambio, M.; Espinosa, A.; Mayer, S. et al. Supplementation with antioxidant-rich extra virgin olive oil prevents hepatic oxidative stress and reduction of de saturation capacity in mice fed a high- fat diet: Effects on fatty acid composition in liver and extrahepatic tissues. Nutrition32(2016) 1254-1267.

Daglaa, I.; Benakia, D.; Baira, E.; Lemonakisb, N.;

Poudyalc,H.; Brownd, L.; Tsarbopoulose, A.; Skaltsounisb, A.L.; Mikrosa, E.; Gikasa, E. Alteration in the liver metabolome of rats with metabolic syndrome after treatment with Hydroxytyrosol. A Mass Spectrometry and Nuclear Magnetic Resonance-based metabolomics study. Talanta 178(2018) 246-257.


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