Characterization and valorization of olive pomace for production of cellulase from Trichoderma reesei RUT C30 in solid-state fermentation

M. Boutiche, F. Sahir-Halouane, L. Meziant, I. Boulaouad, F. Saci, S. Fiala, A. Bekrar, R. Mesbah, A. Abdessemed


Abstract: The aim of the present work is to characterize the olive pomace (physico-chemical and microbiological properties) of four regions known by their high production of olive oil in the north of Algeria and to valorize the lignocellulosic biomass as culture substrate for cellulases production using Trichoderma reesei RUT C-30. The results obtained show that the olive pomace is a favorable environment for the growth of fungi. The load of total aerobic mesophilic flora, yeasts and molds varies from 0.45×106 to 1.36×108 CFU/g DM and from 2×106 to 4.76×107 CFU/g DM, respectively. Physico-chemical parameters of the four regions revealed significant differences (p<0.05). The determination of filter paper activities of enzymatic extracts reveals that olive pomace from Jijel presented the best activity (0.78 U/gds).

Full Text:



Coimbra, M.A.; Cardoso, S.M.; Lopes da Silva, J.A. Olive Pomace, a Source for Valuable Arabinan-Rich Pectic Polysaccharides. In Carbohydrates in Sustainable Development I, Rauter, A.; Vogel, P.; Queneau, Y., Eds.; Springer: Berlin, Germany, 2010; Vol 294, pp. 129-141.

Stamatelatou, K.; Blika, B.S.; Ntaikou, L.; Lyberatos, G. Integrated Management Methods for the Treatment and/or Valorization of Olive Mill Wastes. In Novel Technologies in Food Science: Their Impacts on Products, Consumer Trends and the Environment; Mc Elhatton, A.; do Amaral Sobral, P., Eds.; Springer: New York, USA, 2012; Vol 7, pp. 65-118.

Aggoun-arhab, M. Caractérisation de la composition en microconstituants des margines issues de la production oléicole et utilisabilité comme complément dans la ration chez la vache laitière. Doctorate thesis. Institut National de l’Alimentation et des Technologies AgroAlimentaire (INATAA), Constantine, Algeria, 2016.

Bilan de la campagne oléicole 2015/2016, « Segment huile d’olive ». Observatoire National des Fiières Agricoles et Agroalimentaires (ONFAA). Juin 2016. Available online (accessed on 26 Mars 2017):

Prior Pinto Oliveira Maria Beatriz, Sílvia Guedes Da Costa Anabela, Da Mota Nunes Maria Antónia, Botelho Pimentel Filipa Alexandra. Olive pomace products, method of production and their uses. US Patent N°: US2019153352 (A1)- 2019-05-23.

Sansoucy, R.; Alibes, X.; Martilotti, F.; Nefzaoui, A.; Zoïopoulos, P. Utilisation des sous-produits dans l’olivier en alimentation animale dans le bassin méditerranéen. In FAO production et santé animale N° 43, pp. 121, Rome, 1984.

Aliakbarian, B.; Casazza, A.A.; Perego, P. Valorization of olive oil solid waste using high pressure–high temperature reactor. Food Chemistry 128 (2011) 704-710.

Roig, A.; Cayuela, M.L.; Sánchez-Monedero, M.A. An overview on olive mill wastes and their valorisation methods. Waste Management 26 (2006) 960-969.

Amic, A. ; Dalmasso, C. Unité de valorisation complète de déchets oléicoles par lombricompostage : Production de produits à haute valeur ajoutée : lombricompost, savon, collagène et lombrics. Responsables du projet: Perraud, I.; Roussos, S. Université Aix-Marseille (AMU), France, 2013.

Gudynaite-Savitch, L.; White, T. C. Fungal Biotechnology for Industrial Enzyme Production: Focus on (Hemi) cellulose Production Strategies, Advances and Challenges. In Gene Expression Systems in Fungi: Advancements and Applications, Fungal Biology; Schmoll, M.; Dattenböck, C., Eds.; Springer: Switzerland, 2016, pp .395-439.

Srivastava, N.; Srivastava, M.; Mishra, P.K.; Gupta, V. K.; Rodriguez-Couto, S.; Manikanta, A.; Ramteke, P. W.; Molina, G. Applications of fungal cellulases in biofuel production: Advances and limitations. Renewable and Sustainable Energy Reviews 82 (2018) 2379-2386.

Kim Keun, Lim Young Hoon, Jang You RI. New fungal strain Lecythophora sp. Yp363 and production method of thermostable cellulases using the strain. Korean Patent N°: KR20130083535 (A)- 2013-07-23.

Imran, M.; Anwar, Z.; Irshad, M.; Asad, M. J.; Ashfaq, H. Cellulase production from species of fungi and bacteria from agricultural wastes and its utilization in industry. Adv Enzyme Res 4 (2016) 44-55.

Van Den Broeck Henriette Catherina; De Graaff Leendert H; Visser Jacob; Van Ooyen Albert J. J. Fungal cellulases. US Patent N°: US6190890 (B1)- 2001-02-20.

Mennane, Z.; Tada, S.; Aki, I.; Faid, M.; Hassani, S.; Salmaoui, S. Physico-chemical and microbiological characterization of the olive residue of 26 traditional oil mills in Beni Mellal (Morroco). Les technologies de laboratoire 5 (2010) 4 -9.

AOAC: Official Methods of Analysis. DC Association of Official Analytical Chemists, 15th ed., Arlington VA.USA. Method 945. 18-B, 1990.

Van Soest, P.J.; Robertson, J.B. Systems of analysis for evaluating fibrous feeds. Cornell University Press, New York, 14853 (USA). Dept. of Animal Science, 1980.

Dubois, M.; Gilles, K.A.; Hamilton, T.K.; Rebers, P.A.; Smith, E. Colorimetric method for determination of sugar and related substances. Analytical Chemistry 28 (1956) 350-356.

Miller, G.H. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31(1959) 426-429.

Ghose, T.K. Measurements of cellulase activities. Pure and Applied Chemistry 59 (1987) 257-268.

Silveira, M. H. L.; Aguiar, R.S.; Siika-Aho, M.; Ramos, L.P. Assessment of the enzymatic hydrolysis profile of cellulosic substrates based on reducing sugar release. Bioresource Technology 151(2014) 392-396.

Bradford, M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72 (1976) 248-254.

Baffi, M.A.; Romo-Sanchez, S.; Ubeda-Iranzo, J.; Briones-Pérez, A.I. Fungi isolated from olive ecosystems and screening of their potential biotechnological use.‎ New Biotechnology 29 (2012) 451-456.

Roussos, S.; Perraud-Gaime, I.; Lakhtar, H.; Aouidi, F., Labrousse, Y.; Belkacem, N.; Macarie, H.; Artaud, J. Valorisation biotechnologique des sous-produits de l'olivierparfermentation en milieu solide. Olive biotechnologique (2009) 293-300.

Yansari, A.T.; Sadeghi, H.; Ansari-Pirsarai, Z.; Mohammad-Zadeh, H. Ruminal dry matter and nutrient degradability of different olive cake by-products after incubation in the rumen using nylon bag technique. International Journal of Agricultural and Biology 9(2007) 439-442.

Neifar, M.; Jaouani, A.; Ayari, A.; Abid, O.; Ben Salem, H.; Boudabous, A.; Najar, T.; Ellouze Ghorbel, R. Improving the nutritive value of Olive Cake by solid-state cultivation of the medicinal mushroom Fomes fomentarius. Chemosphere 91 (2013)110-114.

Hendriks, A.T.W.M.; Zeeman, G. Pretreatments to enhance the digestibility of lignocellulosic biomass. Bioresource Technology 100 (2009) 10-18.

Niaounakis, M.; Halvadakis, C.P. Olive-mill waste management: literature review and patent survey. Elsevier, France, 2006.

Sun, W.C.; Cheng, C.H.; Lee, W.C. Protein expression and enzymatic activity of cellulases produced by Trichoderma reesei Rut C-30 on rice straw. Process Biochemistry 43 (2008)1083-1087.

Dhillon, G.S.; Oberoi, H.S.; Kaur, S.; Bansal, S.; Brar, S.K. Value-addition of agricultural wastes for augmented cellulase and xylanase production through solid-state tray fermentation employing mixed-culture of fungi. Industrial Crops and Products 34 (2011) 1160 -1167.

Pirota, R.D.P.B.; Delabona, P.S.; Farinas, C.S. Simplification of the Biomass to Ethanol Conversion Process by Using the Whole Medium of Filamentous Fungi Cultivated Under Solid-State Fermentation. Bioenergy Research 7 (2014) 744 -752.

Abo-State, M.A.M.; Hammad, A.I.; Swelim, M.; Gannam, R.B. Enhanced production of cellulase by Aspergillus spp. isolated from agriculture wastes by solid state fermentation. American-Eurasian Journal of Agricultural & Environmental Sciences 8 (2010) 402-410.


  • There are currently no refbacks.