Characterization by spectrometric methods of chitin produced from white shrimp shells of Parapenaeus longirostris byLactobacillus helveticus cultivated on glucose or date waste

W. Arbia, L. Arbia, L. Adour, A. Amrane, A. Benhadji, H. Lounici


Abstract: Chitin recovery by lactic acid fermentation was considered. It was previously shown that the use of glucose led to 61% of demineralization and 42% of deproteinization; while and unlike glucose, the inoculation of L. helveticus in date waste (200 g L-1 reducing sugars) led to a simultaneous demineralization and deproteinization of shrimp shells, 83 and 61 %, respectively. The purity of end-products were characterized by infrared (FTIR), X-ray diffraction and X-ray fluorescence.

Analysis of the chemical composition of fermentation end-product by X-ray fluorescence showed that residual minerals are in trace except CaO and P2O5. The infrared spectra and the X-ray diffractograms of both samples compared to those of pure chitin showed a strong similarity. FTIR of fermented shells showed the appearance of characteristic peaks of α-chitin and the disappearance of calcite peaks. In addition and especially for the shells fermented using L. helveticus cultivated on date waste, a similarity with those of pure chitin should be noted. These results confirmed the efficiency of biological chitin recovery, and that a synthetic carbon source (glucose) can be replaced by a natural one, date waste, allowing a valorization of this latter. The relevance of subsequent works to improve yields of demineralization and deproteinization was therefore confirmed.

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