Anti-inflammatory activity of different extracts from Laurus nobilis growing in Algeria

R. Guedouari, M. Nabiev


Abstract: Laurus nobilis was a common plant raised in Algeria and various researches were confirmed the medicinal potential of this plant. Some studies were focused on the antioxidant and antimicrobial properties of this plant but no studies were performed to determine its anti-inflammatory activity in vivo. Thus, the present study was carried out to study and compare the anti-inflammatory effect of the ethanolic extract of leaves with that of the fixed oil extracted from the fruits of Laurus nobilis. For our experiment, male BALB / c mice (n = 40) weighing 20 ± 1.0 g at 8 weeks old were divided into four treatment groups (10 mice / group): (a) control, (b) extract fruit (fixed oil), (c) leaf extract (ethanolic extract 100 mg / kg) and (d) reference (Diclofenac 50 mg). These mice were housed under standard environmental conditions. Anti-inflammatory capacity was evaluated by hind paw oedema model using carrageenan-induced inflammation in mice. The ethanolic leaf extract reduced the increase in the volume of the paw with a percentage inhibition of 30.62% (p <0.05), this percentage was found to be 26.33% (p <0.05 ) with fixed oil from Laurus nobilis fruit, while the activity of Diclofenac was 17.59% (p <0.05). In conclusion, the importance of Laurus nobilis lay in their fixed oil and their ethanolic extract which could be used scientifically in the treatment of inflammation. A phytochemical screening of the leaves and fruits of Laurus nobilis showed the presence of flavonoids and saponins which may be at the origin of the anti-inflammatory properties of the extract. The total phenolic in the leaf extract of  Laurus nobilis  was  37,72±0,336 μg GAE /mg of dry extract.

Full Text:



Kilic, A.; Altuntas, E. Wood and bark volatile compounds of Laurus nobilis. Holz als Roh- und Werkstoff 64(2006) 317.

Politeo, O.; Jukic, M.; Milos, M. Chemical composition and antioxidant activity of free volatile aglycones from laurel (Laurus nobilis L.) compared to its essential oil. Croatica chemical Acta 80(2007)121-126.

Sayyah, M.; Saroukhani, G.; Peirovi, A.; Kamalinejad, M. Analgesic and anti-inflammatory activity of the leaf essential oil of Laurus nobilis Linn. Phytotherapy Research 17(2003)733-736.

Derwich, E.; Benziane, Z.; Boukir, A. Chemical composition and antibacterial activity of leaves essential oil of Laurus nobilis from morocco. Australian Journal of Basic and Applied Sciences 3(2009)3818-3824.

Khalil, E.; Affifi, F.; Al-Hussaini, M. Evaluation of the wound healing effect of some jordanian traditional medicinal plants formulated in pluronic F127 using mice ( Mus musculus). Journal of Ethnopharmacology 109(2007)104-112.

Nourbakhsh, M.; Bal, Y. Recovery of fixed and volatile oils from Laurus nobilis fruit and leaves by solvent extraction method. Engineering & Architecture Faculty Eskişehir Osmangazi University 18(2005)15-24.

Alhakmani, F.; Sokindra, K.; Shah, A.K. Estimation of total phenolic content in–vitro antioxidant and anti–inflammatory activity of flowers of Moringa oleifera. Asian Pacific Journal of Tropical Biomedicine 3(2013) 623-627.

Shokoohinia, Y.; Yegdaneh, A.; Amin, G.; Ghannadi, A. Seasonal variations of Laurus nobilis leaves volatile oil components in Isfahan. Research Journal of Pharmacognosy 1(2014)1-6.

Torki, A.; Khalaji-Pirbalouty, V. Anchusa italic extract: phytochemical and neuroprotective evaluation on global cerebral ischemia and reperfusion. Brazilian Journal of Pharmaceutical Sciences 54(2018) e17251.

Christy, JE.; Justin, J.P.T. In vitro control of staphylococcus aureus (NCTC 6571) and Escherichia coli (ATCC 25922) by ricinus communis L. Asian Pacific Journal of Tropical Biomedicine 2(2012)717-721.

Mahadeva-rao, US.; Abdurrazak, M.; Suryati mohd, K. Phytochemical screening, total flavonoid and phenolic content assays of various solvent extracts of tepal of musa paradisiacal. Malaysian Journal of Analytical Sciences 20(2016)1181-1190.

Merouane, A.; Noui, A.; Medjahed, H.; Nedjari Benhadj Ali, K.; Saadi, A. Activité antioxydante des composes phénoliques d’huile d’olive extraite par méthode traditionnelle. International Journal of Biological and Chemical Sciences 8 (2015)1865-1679.

Colot, M. Notions techniques de pharmacologie générale. Masson & Cie Editeurs (1972) 137.

Amezouar, F.; Badri, W.; Hsaine, M.; Bourhim, N.; Fougrach, H. Évaluation des activités antioxydante et anti-inflammatoire de Erica arborea L. du Maroc. Pathologie Biologie 61(2013) 254-258.

Soro, T.Y.; Néné-bi, A.S.; Zahoui, O.S.; Yapi, A.; Traoré, F. Activité anti- inflammatoire de l’extrait aqueux de Ximenia americana (Linné) (Olacaceae). Journal of Animal and Plant Sciences 24(2015) 3802-3813.

Kamal, R.; Kharbach, M.; Eljemli, M.; Bouklouze, A.; Cherrah, Y.; Alaoui, K . Activité anti-inflammatoire in vivo de l’huile d’argan. Phytothérapie (2019) 1-7.

Etame loe, G.; Ngaba, G.P.; Kamdom, M.; Mpondo, E. M.; Dibong, S. D. Evaluation des activités anti-inflammatoire et antiradicalaire de l’extrait au vin de palme des feuilles de Phragmanthera capitata (Sprengel) S. Balle (Loranthaceae) récoltées sur Psidium guajava au Cameroun. International Journal of Biological and Chemical Sciences 12(2018) 233-243.

Cowan, M.M. Plant products as antimicrobial agents. Clinical Microbiology Reviews 12(1999) 564-582.

Onwuliri, F.C.; Wonang, D.L. Studies on the combined antibacterial action of Ginger (Zingiber officinale L) and Garlic ( Allium sativum L) on some bacteria. Nigerian Journal of Botany 18(2005) 224-228.

Diatta, W.; Guata Yoro, S.Y.; Manga, C.I.; Diatta, K.; Fall, A.D.; Bassene, E. Recherche des activités anti-inflammatoire et analgésique des extraits de feuilles de Zanthoxylum zanthoxyloides (Lam) zepernick et timler (Rutaceae) . International Journal of Biological and Chemical Sciences 8(2014)128-133.

Hongyan, L.; Zeyuan, D.; Ronghua, L.; Steven, L.; Rong, T. Bioaccessibility, in vitro antioxidant activities and in vivo anti-inflammatory activities of a purple tomato (Solanum lycopersicum L.). Food Chemistry 159(2014) 353-360.

Rahmani, S.; Belboukhari, N.; Sekkoum, K.; Cheriti, A. Evaluation de l'activité anti-inflammatoire d’extraits aqueux de feuilles Limoniastrum feei (plumbaginacea).Algerian journal of arid environment 6 (2016 ) 80-86.

Yougbaré-Ziébrou, M.N.; Ouédraogo, N.; Lompo, M.; Bationo, H.; Yaro, B.; Gnoula, C.; Sawadogo, W.R.; Guissou, I.P. Activités anti-inflammatoire, analgésique et antioxydante de l’extrait aqueux des tiges feuillées de Saba senegalensis Pichon (Apocynaceae) Anti-inflammatory, analgesic and antioxidant activities of an aqueous extract of Saba senegalensis Pichon stems with leaves (Apocynaceae). Phytothérapie (2015)1-7.

Sangita, C.; Priyanka, C.; Protapaditya, D.; Sanjib, B. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pacific Journal of Tropical Biomedicine 2(2012)S178-S180.


  • There are currently no refbacks.