Detection of extended-spectrum beta-lactamases in Gram-negative bacilli from seawater in Algiers port (Algeria)

S. Alouache, Y. Messai, L. Anssour, H. Iabadene, G. Arlet, R. Bakour

Abstract


The antibiotics resistance of aquatic environmental bacteria is an established fact; however, in order to understand its origin and development, it is necessary to elucidate its mechanisms. Basing on the importance of beta-lactams in therapy and extended-spectrum beta-lactamases (ESBLs) as a resistance mechanism, the objective of this work was to investigate ESBLs in Gram negative bacilli recovered from seawaters of Algiers port. Twelve isolates, identified as Citrobacter freundii (n=4), Escherichia coli (n=1), Aeromonas hydrophila (n=2), Vibrio fluvialis (n=4) and Bordetella sp (n=1), were found producers of ESBLs  CTX-M-3, CTX-M-15, PER-1, VEB-1, SHV2a and oxacillinase OXA-101. blaCTX-M and  blaPER-1 genes were transferred in association with self-transferable plasmids of approximately >70kb and 138 kb, respectively. The class - integrons were present in 7 isolates and were transferable from 3 isolates. The insertion sequence ISEcp1B was found genetically linked to blaCTX-M genes. This study described different types of ESBLs in marine water which is related to anthropogenic inputs. This is an emerging concern around the world and requires implementation of serious resistance monitoring in environment.


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Hawkey, P.M.; Jones, A.M. The changing epidemiology of resistance. Journal of Antimicrobial Chemotherapy 64(Suppl. 1) (2009) i3-10.

Kümmerer, K. Resistance in the environment. Journal of Antimicrobial Chemotherapy 54 (2004) 311- 320.

Maravić, A.; Šamanić, I.; Šprung, M.; Fredotović, Ž.; Ilić, N.; Dragičević, J.; Puizina, J. Broad-spectrum resistance of Pseudomonas aeruginosa from shellfish: infrequent acquisition of novel resistance mechanisms. Environmental Monitoring and Assessment 190 (2018) 81.

Nappier, S.P.; Liguori, K.; Ichida, A.M.; Stewart, J.R.; Jones, K.R. Antibiotic Resistance in Recreational Waters: State of the Science. International Journal of Environmental Research and Public Health 17 (2020) 8034.

Davies, J.; Davies, D. Origins and Evolution of Antibiotic Resistance. Microbiology and Molecular Biology Reviews 74(2010) 417-433.

Goni-Urriza, M.; Capdepuy, M.; Arpin, C.; Raymond, N.; Caumette, P.; Quentin, C. Impact of an Urban Effluent on Antibiotic Resistance of Riverine Enterobacteriaceae and Aeromonas spp. Applied and Environmental Microbiology 66 (2000) 125–132.

Guardabassi, L.; Wong, D.M.A.Lo.Fo.; Dalsgaard, A. The effects of tertiary wastewater treatment on the prevalence of antimicrobial resistant bacteria. Water Research 36 (2000)1955–1964.

Alouache, S.; Kada, M.; Messai, Y.; Estepa, V.; Torres, C.; Bakour, R. Antibiotic Resistance and Extended-Spectrum β-Lactamases in Isolated Bacteria from Seawater of Algiers Beaches (Algeria). Microbes and Environments 27(2012) 80–86.

Maravić, A.; Skočibušić, M.; Cvjetan, S.; Šamanić, I.; Fredotović, Ž.; Puizina, J. Prevalence and diversity of extended-spectrum-β-lactamase-producing Enterobacteriaceae from marine beach waters. Marine Pollution Bulletin 90 (2015) 60-67.

CA-SFM : Comité de l’Antibiogramme de la Société Française de Microbiologie. Communiqué 2007. Société Française de Microbiologie. http://www.sfm.asso.fr/

Jarlier, V.; Nicolas, M.; Fournier, G.; Philippon, A. Extended broad- spectrum beta-lactamases conferring transferable resistance to newer β-lactam agents in Enterobacteriaceae: hospital prevalance and susceptibility patterns. Reviews of Infectious Diseases 4 (1988)143-149.

Decré, D.; Burghoffer, B.; Gautier, V.; Petit, J.C.; Arlet, G. Outbreak of multiresistant Klebsiella oxytoca involving strains with extended-spectrum β-lactamase. Journal of Antimicrobial Chemotherapy 54(2004) 881-888.

Kado, C.I.; Liu, S.T.; Rapid procedure for detection and isolation of large and small plasmids. Journal of Bacteriology 45 (1981) 1365-1373.

Kim, J.; Park, Y.J.; Kim, S.I.; Kang, M.W.; Lee, S.O.; Lee, K.Y. Nosocomial outbreak by Proteus mirabilis producing extended-spectrum β-lactamase VEB-1 in a

Korean university hospital. Journal of Antimicrobial Chemotherapy 54 (2004) 1144-1147.

Messai, Y.; Iabadene, H.; Benhassine, T.; Alouache, S.; Tajui, M.; Gautier, V.; Arlet, G.; Bakour., R. Prevalence and characterisation of extended-spectrum beta-lactamases in Klebsiella pneumoniae in Algiers hospitals (Algeria). Pathologie Biologie 56 (2008) 319-325.

Di Conza, J.; Ayala, J.A.; Power, P.; Mollerach, M.; Gutkind, G. Novel class 1 integron (In S21) carrying blaCTX-M-2 in Salmonella enterica serovar Infantis. Antimicrobial Agents and Chemotherapy 46 (2002) 2257–2261.

Murinda, S.E.; Ebner, P.D.; Nguyen, L.T.; Mathew, A.G.; Oliver, S.P. Antimicrobial resistance and class 1 integrons in pathogenic Escherichia coli from dairy farms. Foodborne Pathogens and Disease 2 (2005) 348–352.

Eckert, C.; Gautier, V.; Arlet, G. DNA sequence analysis of the genetic environment of various blaCTX-M genes. Journal of Antimicrobial Chemotherapy 57(2006)14–23.

Poirel, L.; Decousser, J.W.; Nordmann, P. Insertion sequence ISEcp1B is involved in expression and mobilization of a blaCTX-M β-lactamase gene. Antimicrobial Agents and Chemotherapy 47 (2003) 2938-45.

Alouache, S.; Estepa, V.; Messai, Y.; Ruiz, E.; Torres, C.; Bakour, R. Characterization of ESBLs and associated quinolone resistance in Escherichia coli and Klebsiella pneumoniae isolates from an urban wastewater treatment plant in Algeria. Microbial Drug Resistance 20 (2014):30-38.

Amarasiri, M.; Daisuke Sano, D.; Suzuki, S. Understanding human health risks caused by antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) in water environments:Current knowledge and questions to be answered. Critical reviews in Environmental Science and Technology. 50(2020) 2016–2059.

Machado, E.; Coque, T.M.; Cantón, R.; Sousa, J.C.; Silva, D.; Ramos, M.; Rocha, J.; Ferreira, H.; Peixe, L. Leakage into Portuguese aquatic environments of extended-spectrum-β-lactamase-producing Enterobacteriaceae. Journal of Antimicrobial Chemotherapy 63 (2009) 616-618.

Kim, J.; Kang, H.Y.; Lee, Y. The identification of CTX-M-14, TEM-52, and CMY-1 enzymes in Escherichia coli isolated from the Han River in Korea. Journal of Microbiology 46 (2008) 478-481.

Martinez, J.L. The role of natural environments in the evolution of resistance traits in pathogenic bacteria. Proceedings of the Royal Society B 276 (2009) 2521-2530.

Iabadene, H.; Messai, Y.; Dallenne, C.; Geneste, D.; Bakour, R.; Arlet, G. Emergence of extended-spectrum β-lactamase PER-1, in Proteus vulgaris and Providencia stuartii from Algiers, Algeria. Antimicrobial Agents and Chemotherapy 53 (2009) 4043-4044.

Jiang, X.; Ni, Y.; Jiang, Y. Outbreak of infection caused by Enterobacter cloacae producing the novel VEB-3 beta-lactamase in China. Journal of Clinical Microbiology 43(2005) 826-831.

Iabadene, H.; Messai, Y.; Ammari, H.; Ramdani-Bouguessa, N.; Lounes, S.; Bakour, R.; Arlet, G. Dissemination of ESBL and Qnr determinants in Enterobacter cloacea in Algeria. Journal of Antimicrobial Chemotherapy 62 (2008) 133-136.

Poirel, L.; Gniadkowski, M.; Nordmann, P. Biochemical analysis of the ceftazidime-hydrolysing extended-spectrum beta-lactamase CTX-M-15 and of its structurally related beta-lactamase CTX-M-3. Journal of Antimicrobial Chemotherapy 50 (2002) 1031–1034.

Donati, V.; Feltrin F.; Hendriksen, R.S.; Svendsen, C.A.; Cordaro, G.; García-Fernández, A.; Lorenzetti, S.; Lorenzetti, R.; Battisti. A.; Franco, A. Extended-Spectrum-Beta-Lactamases, AmpC Beta-Lactamases and Plasmid Mediated Quinolone Resistance in Klebsiella spp. from Companion Animals in Italy. PLoS One 9 (2014) e90564.

Girlich, D.; Poirel, L.; Nordmann, P. Diversity of Clavulanic Acid-Inhibited Extended-Spectrum beta-lactamases in Aeromonas spp. from the Seine River, Paris, France. Antimicrobial Agents and Chemotherapy 55 (2011) 1256–1261.

Porto, A.; Ayala, J.; Gutkind, G.; Di Conza, J. A. novel OXA-10–like β-lactamase is present in different Enterobacteriaceae. Diagnostic Microbiology and Infectious Disease 66 (2010) 228-29.

Dolejska, M.; Bierosova, B.; Kohoutova, L.; Literak, I.; Cizek, A. Antibiotic-resistant Salmonella and Escherichia coli isolates with integrons and extended-spectrum beta-lactamases in surface water and sympatric black-headed gulls. Journal of Applied Microbiology 106 (2009)1941-1950.


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