Biodegradation of Petroleum hydrocarbons and Biosurfactant production by an extremely halophilic Archaea Halovivax sp. A21

S. Khemili-Talbi, S. Kebbouche-Gana, S. Akmoussi-Toumi, M.L. Gana, S. Lahiani, Y. Angar, I. Ferrioune

Abstract


Abstract: Over the recent few years, biosurfactant has played an important role in the industrial application especially in oil recovery; even in high salinity conditions. The potential of biosurfactant production by the extreme halophilic archaeon Halovivax sp. A21 in the presence of petroleum hydrocarbons (2% v/v) as sole carbon source at high salinity (25% NaCl) has been investigated. The results show the ability of Halovivax sp. A21 to grow and reduce surface tension under an optimum range of pH (7-9), salinities (15-35% NaCl) and temperature (40-45°C) for an optimized volume of 100 ml of the medium for 1000 ml capacity Erlenmeyer flasks with an optimum agitation of 120 rpm. The rates of biosurfactant production on petroleum hydrocarbons were enhanced with increasing NaCl concentration in the medium with an optimum of 25%.  Biosurfactant production by Halovivax sp. A21 showed high emulsifying activity (more than 80%) and decreased surface tension (24.5 mN/m). The stability of the produced biosurfactant was determined by different physico-chemical conditions like pH, temperature and salinity. Moreover, the partial purification of the derived biosurfactant by silica gel column chromatography and Thin-layer chromatography revealed that it belongs to the lipopeptide group. Although both catechol dioxygenases participated in the degradation of petroleum hydrocarbons, more induction of catechol 1,2 dioxygenase was observed than the catechol 2,3 dioxygenase which indicated the predominance of the ortho cleavage pathways in the petroleum hydrocarbons degradation by the halophilic strain Halovivax sp. A21. The results demonstrated that strain Halovivax sp. A21 was able to increase the bioavailability of insoluble hydrocarbons, thus facilitating their uptake and their biodegradation even at high salt concentration. Likewise, the search of novel biosurfactants in extremophiles, or the use of microorganisms that present excellent degradation capacity together with the production of stable biosurfactant from contaminant (hydrocarbon compounds) as a carbon source seem to be particularly promising since they have particular adaptations to increase stability in adverse extreme environments.


Full Text:

PDF

References


Chikere C.B., Obieze C.C., Okerentugba P., Molecular Assessment of Microbial Species Involved in the Biodegradation of Crude Oil in Saline Niger Delta Sediments Using Bioreactors, J Bioremed Biodeg 6 (2015) 307.

Corsellis Y., Krasovec M., Sylvi L., Cuny P., Militon C., Oil removal and effects of spilled oil on active microbial communities in close to salt-saturation brines, Extremophiles 20(3) (2016) 235-50.

Fathepure B.Z., Recent studies in microbial degradation of petroleum hydrocarbons in hypersaline environments, Front Microbiol 5 (2014) 1–16 .

Kebbouche-Gana S., Gana M.L., Ferrioune I., Khemili S., Lenchi N., Akmouci-Toumi S., Bouanane-Darenfed N.A., Djelali N.D., Production of biosurfactant on crude date syrup under saline conditions by entrapped cells of Natrialba sp. strain E21, an extremely halophilic bacterium isolated from a solar saltern (Ain Salah, Algeria), Extremophiles 17 (2013) 981–993.

Paniagua-Michel J., Rosales A., Marine Bioremediation - A Sustainable Biotechnology of Petroleum Hydrocarbons Biodegradation in Coastal and Marine Environments, J Bioremed Biodeg 6 (2015) 273.

Bonete M.J., Bautista V., Esclapez J., García-Bonete M.J., Pire C., CamachoM. , Torregrosa-Crespo J., Martínez-Espinosa R.M., New Uses of Haloarchaeal Species in Bioremediation Processes, Advances in Bioremediation of Wastewater and Polluted Soil, Prof. Naofumi Shiomi (Ed.), InTech, (2015), DOI: 10.5772/60667.

Bertrand J.C., Almallah M., Acquaviva M., Mille G., Biodegradation of hydrocarbons by an extremely halophilic archaebacterium, Lett Appl Microbiol 11 (1990) 260–263

Kulichevskaya I.S., Milekhina E.I., Borzenkov I.A., Zvyagintseva I.S., Belyaev S.S., Oxidation of petroleum hydrocarbons by extremely halophilic archaebacteria, Microbiology 60(2) (1992) 596-601.

Oren A., Gurevich P., Azachi M., Hents Y., Microbial degrada- tion of pollutants at high salt concentrations, Biodegradation 3 (1992) 387–398.

Emerson D., Chauhan S., Oriel P., Breznak JA., Haloferax sp. D1227: a halophilic archaeon capable of growth on aromatic compounds, Arch Microbiol 161(6) !1994) 445-452.

Margesin R., Schinner F., Biodegradation and bioremediation of hydrocarbons in extreme environments., Appl Microbiol Biotechnol 56(5-6) (2001) 650-663.

Garcia, M. T., Mellado, E., Ostos, J. C., and Ventosa, A., Halomonas organivorans sp. nov., a moderate halophile able to degrade aromatic compounds, Int. J. Syst. Evol. Microbiol. 54 (2004) 1723–1728.

Nicholson C.A., Fathepure B.Z., Biodegradation of benzene by halophilic and halotolerant bacteria under aerobic conditions, Appl Environ Microbiol 70 (2004) 1222–1225.

Zhao B., Wang H., Mao X., Li R., Biodegradation of phenanthrene by a halophilic bacterial consortium under aerobic conditions, Curr Microbiol 58 (2009) 205–210

Sei A., Fathepure B. Z., Biodegradation of BTEX at high salinity by an enrichment culture from hypersaline sediments of Rozel Point at Great Salt Lake. J. Appl. Microbiol. 107 (2009) 2001–2008.

Al-Mailem D.M., Sorkhoh N. A., Al-Awadhi H., Eliyas M., Radwan, S. S., Biodegradation of crude oil and pure hydrocarbons by extreme halophilic archaea from hypersaline coasts of the Arabian Gulf. Extremophiles 14, (2010) 321–328.

Al-Mailem D.M., Eliyas M., Radwan S.S., Oil-bioremediation potential of two hydrocarbonoclastic, diazotrophic Marinobacter strains from hypersaline areas along the Arabian Gulf coasts, Extremophiles 17 (2013) 463–470.

Al-Mailem, D. M., Eliyas, M., and Radwan, S. S., Enhanced haloar- chaeal oil removal in hypersaline environments via organic nitrogen fertilization and illumination. Extremophiles 16 (2012) 751–758.

Al-Mailem D.M., Eliyas M., Khanafer M., Radwan S.S., Culture- dependent and culture-independent analysis of hydrocarbonoclastic microorganisms indigenous to hypersaline environments in Kuwait. Microb Ecol 67 (2014) 857-865.

Tapilatu Y.H., Grossi V., Acquaviva M., Militon C., Bertrand J.C., Cuny P., Isolation of hydrocarbon degrading extremely halophilic Archaea from an uncontaminated hypersaline pond (Camargue, France), Extremophiles 14 (2010) 225–231.

Bonfá M.R.L., Grossman M.J., Mellado E., Durrant L.R., Biodegradation of aromatic hydrocarbons by Haloarchaea and their use for the reduction of the chemical oxygen demand of hypersaline petroleum produced water, Chemosphere 84 (2011) 1671–1676.

Dastgheib S.M.M., Amoozegar M.A., Khajeh K., Shavandi M., Ventosa A., Biodegradation of polycyclic aromatic hydrocarbons by a halophilic microbial consortium. Appl Microbiol Biotechnol 95 (2012) 789–798 .

Dalvi S., Azetsu S., Patrauchan M.A., Aktas D.F., Fathepure B.Z., Proteogenomic elucidation of the initial steps in the benzene degradation pathway of a novel halophile, Arhodomonas sp. Strain Rozel, isolated from a hypersaline environment, Appl Environ Microbiol 78 (2012) 7309–7316.

Erdoğmus S.F., Mutlu B., Korcan S.E., Guven K., Konuk M., Aromatic hydrocarbon degradation by halophilic archaea isolated from Çamalti Saltern, Turkey, Water Air Soil Pollut 224 (2013) 1449.

Khemili-Talbi S., Kebbouche-Gana S., Akmoussi-Toumi S., Angar Y., Gana M.L., Isolation of an extremely halophilic arhaeon Natrialba sp. C21 able to degrade aromatic compounds and to produce stable biosurfactant at high salinity, Extremophiles, 19(6) (2015) 1109-20.

Reineke, W. (2001). “Aerobic and anaerobic biodegradation potentials of microor- ganisms,” in The Handbook of Environmental Chemistry. Vol. 2 Part K Biodegradation and Persistance, ed B. Beek (Berlin; Heidelberg: Springer- Verlag), 1–140.

Van Hamme J.D., Singh A.M., Ward O.P. Recent advances in petroleum microbiology, Microbiol. Mol. Biol. Rev. 6 (2003) 503–549.

Pérez-Pantoja D., González B., Pieper D. H., Aerobic degradation of aromatic hydrocarbons, in Handbook of Hydrocarbon and Lipid Microbiology, ed K. N. Timmis (Berlin: Springer-Verlag), (2010) 799–837.

Kumari B., Singh S.N., Singh D.P., Characterization of two biosurfactant producing strains in crude oil degradation. Process Biochem 47 (2012) 2463–2471.

Sarafin Y., Donio M.B., Velmurugan S., Michaelbabu M., Citarasu T. Kocuria marina BS-15 a biosurfactant producing halophilic bacteria isolated from solar salt works in India. Saudi J Biol Sci 21(6) (2014) 511–519.

Selim S.A., El-Alfy S.M., Hagagy N.I., Hassanin A.AI., Khattab R.M., El-Meleigy E.A., Abdel Aziz1 M.H., Maugeri T.L., Oil-biodegradation and biosurfactant production by haloalkaliphilic Archaea isolated from Soda lakes of the Wadi An Natrun, Egypt. Journal of Pure and Applied Microbiology, 6 (3) (2012) 1011-1020.

Kebbouche-Gana S., Gana M. L., Khemili S., Fazouane-Naimi F., Bouanane N. A., Penninckx M., Hacene H., Isolation and characterization of halophilic Archaea able to produce biosurfactants, J Ind Microbiol Biotechnol 36 (2009) 727-738.

Fu W., Oriel P. Degradation of 3-phenylpropionic acid by Haloferaxsp. D1227., Extremophiles 3 (1999) 45–53

Arulazhagan P., Vasudevan N., Role of a moderately halophilic bacterial consortium in the biodegradation of polyaromatic hydrocarbons. Mar Pollut Bull 58(2) (2009) 256–262.

Cooper D. G., Goldenberg B. G., Surface active agents from two Bacillus species, Appl Environ Microbiol 53 (1987) 224-229.

Maneerat S., Phetrong K., Isolation of biosurfactant-producing marine bacteria and characteristics of selected biosurfactant, J Sci Technol 29 (2007) 781- 791.

Das M., Das S.K., Mukherjee R.K. Surface active properties of the culture filtrates of a Micrococcus species grown on n-alkenes and sugars, Bioresour Technol, 63 (1998) 231–235

Garcia M.T., Ventosa A., Mellado E., Catabolic versatility of aromatic compound degrading halophilic bacteria. FEMS Microbiol Ecol 54 (2005) 97–109.

Das P., Mukherjee S., Sen R., Substrate dependent production of extracellular biosurfactant by a marine bacterium, Bioresource Technology 100 (2009) 1015–1019.

Mulligan C.N., Environmental applications for biosurfactants, Environ Poll 133 (2005) 183-198.

Gnanamani A., Kavitha V., Radhakrishnan N., Mandal A.B., Bioremediation of Crude Oil Contamination Using Microbial Surface-Active Agents: Isolation, Production and Characterization, J Bioremed Biodegrad 1 (2010) 107.

Mohajeri L., Aziz H.A., Isa M.H., Zahed M.A., Mohajeri S., Ex-situ bioremediation of crude oil in soil, a comparative kinetic analysis, Bull Environ Contam Toxicol 85 (2010) 54

Martinez-Checa F., Toledo F.L., Vilchez R., Quesada E., Calvo C (2002) Yield production, chemical composition and functional properties of emulsifier H28 synthesized by Halomonas eurihalina strain H-28 in media containing hydrocarbons, Appl Microb Biotechnol 58 (2002) 358-63.

Elazzazy A.M., Abdelmoneim T.S., Almaghrabi O.A., Isolation and characterization of biosurfactant production under extreme environmental conditions by alkali-halo-thermophilic bacteria from Saudi Arabia, Saudi Journal of Biological Sciences 22 (2015) 466–475.

Post F.J., Al-Harjan F.A., Surface activity of Halobacteria and potential use in microbial enhanced oil recovery. Sys Appl Microbiol 11 (1988) 97-101.

Anton J., Meseguer I., Rodriguez-Valera F., Production of an Extracellular Polysaccharide by Haloferax mediterranei, Appl

Djeridi I., Militon C., Grossi V., Cuny P., Evidence for surfactant production by the haloarchaeon Haloferax sp. MSNC14 in hydrocarbon-containing media, Extremophiles 17 (2013) 669–675.


Refbacks

  • There are currently no refbacks.