Detection of extended-spectrum beta-lactamases in Gram-negative bacilli from seawater in Algiers port (Algeria)

S. Alouache, Y. Messai, L. Anssour, H. Iabadene, G. Arlet, R. Bakour


Abstract: The antibiotics resistance of aquatic environmental bacteria is an established fact; however, in order to understand its origin and development, it is necessary to elucidate its mechanisms. Basing on the importance of beta-lactams in therapy and extended-spectrum beta-lactamases (ESBLs) as a resistance mechanism, the objective of this work was to investigate ESBLs in Gram negative bacilli recovered from seawaters of Algiers port. Twelve isolates, identified as Citrobacter freundii (n=4), Escherichia coli (n=1), Aeromonas hydrophila (n=2), Vibrio fluvialis (n=4) and Bordetella sp (n=1), were found producers of ESBLs  CTX-M-3, CTX-M-15, PER-1, VEB-1, SHV2a and oxacillinase OXA-101. blaCTX-M and  blaPER-1 genes were transferred in association with self-transferable plasmids of approximately >70kb and 138 kb, respectively. The class - integrons were present in 7 isolates and were transferable from 3 isolates. The insertion sequence ISEcp1B was found genetically linked to blaCTX-M genes. This study described different types of ESBLs in marine water which is related to anthropogenic inputs. This is an emerging concern around the world and requires implementation of serious resistance monitoring in environment.

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